Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF

Horst Funken; Kai Malte Bartels; Susanne Wilhelm; Melanie Brocker; Michael Bott; Manjeet Bains; Robert E.W. Hancock; Frank Rosenau; Karl Erich Jaeger

doi:10.1371/journal.pone.0046857

Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF

Horst Funken, Kai Malte Bartels, Susanne Wilhelm, Melanie Brocker, Michael Bott, Manjeet Bains, Robert E.W. Hancock, Frank Rosenau, Karl Erich Jaeger

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.

Original language	English
Article number	e46857
Journal	PloS one
Volume	7
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0046857
Publication status	Published or Issued - 8 Oct 2012
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
General Agricultural and Biological Sciences
General

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abstract = "The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.",

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AU - Funken, Horst

AU - Bartels, Kai Malte

AU - Wilhelm, Susanne

AU - Brocker, Melanie

AU - Bott, Michael

AU - Bains, Manjeet

AU - Hancock, Robert E.W.

AU - Rosenau, Frank

AU - Jaeger, Karl Erich

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AB - The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.

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Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF

Abstract

ASJC Scopus subject areas

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