TY - JOUR
T1 - Structure, organization, and chromosomal mapping of the human neurogranin gene (NRGN)
AU - De Arrieta, Cruz Martínez
AU - Jurado, Luis Pérez
AU - Bernal, Juan
AU - Coloma, Antonio
N1 - Funding Information:
We thank Elena Candel for expert technical assistance and Dr. M. Cervera for critically reading the manuscript. This work was supported by a grant from DGICYT (Spain). C.M. is a recipient of a predoctoral fellowship from Gobierno Vasco. L.P.J. is a recipient of a research contract from the Spanish Ministry of Education.
PY - 1997/4/15
Y1 - 1997/4/15
N2 - In this report the identification, structure, and chromosomal localization of the human neurogranin gene (NRGN) are described. NRGN is the human homolog of the rat Ng/RC3 gene, which encodes a brain-specific protein expressed in telencephalic neurons. The human NRGN gene spans approximately 12 kb and contains four exons and three introns. All splice acceptor and donor sites conform to the canonical AG/GT rule. Human neurogranin sequence predicts a 78-amino-acid protein with 5 amino acids encoded by exon 1 and the remaining 73 amino acids encoded by exon 2. The third and fourth exons contain untranslated sequences. The overall degree of homology between the human and the rat coding sequences is 90% for the nucleic acid sequence, with 96% identity and 97.5% similarity at the protein level. The NRGN gene is expressed exclusively in brain as a single 1.3-kb mature mRNA. The promoter lacks both TATA and CAAT boxes, but shows a consensus sequence for an initiator element located 234 bases upstream from the AUG initiation codon. The 5'-flanking region contains multiple putative binding sites for transcription factors such as Sp1, GCF, AP2, and PEA3. Analysis of a panel of radiation hybrids has led to localization of the NRGN gene in YAC 763A2 (CEPH), previously mapped at 11q24. This locus is contained in a region of conserved synteny with mouse chromosome 9.
AB - In this report the identification, structure, and chromosomal localization of the human neurogranin gene (NRGN) are described. NRGN is the human homolog of the rat Ng/RC3 gene, which encodes a brain-specific protein expressed in telencephalic neurons. The human NRGN gene spans approximately 12 kb and contains four exons and three introns. All splice acceptor and donor sites conform to the canonical AG/GT rule. Human neurogranin sequence predicts a 78-amino-acid protein with 5 amino acids encoded by exon 1 and the remaining 73 amino acids encoded by exon 2. The third and fourth exons contain untranslated sequences. The overall degree of homology between the human and the rat coding sequences is 90% for the nucleic acid sequence, with 96% identity and 97.5% similarity at the protein level. The NRGN gene is expressed exclusively in brain as a single 1.3-kb mature mRNA. The promoter lacks both TATA and CAAT boxes, but shows a consensus sequence for an initiator element located 234 bases upstream from the AUG initiation codon. The 5'-flanking region contains multiple putative binding sites for transcription factors such as Sp1, GCF, AP2, and PEA3. Analysis of a panel of radiation hybrids has led to localization of the NRGN gene in YAC 763A2 (CEPH), previously mapped at 11q24. This locus is contained in a region of conserved synteny with mouse chromosome 9.
UR - http://www.scopus.com/inward/record.url?scp=0031569831&partnerID=8YFLogxK
U2 - 10.1006/geno.1997.4622
DO - 10.1006/geno.1997.4622
M3 - Article
C2 - 9143500
AN - SCOPUS:0031569831
SN - 0888-7543
VL - 41
SP - 243
EP - 249
JO - Genomics
JF - Genomics
IS - 2
ER -