Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9

Fatwa Adikusuma; Nicole Williams; Frank Grutzner; James Hughes; Paul Thomas

doi:10.1016/j.ymthe.2017.05.021

Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9

Fatwa Adikusuma, Nicole Williams, Frank Grutzner, James Hughes, Paul Thomas

Gene Editing

Research output: Contribution to journal › Letter › peer-review

64 Citations (Scopus)

Abstract

CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.

Original language	English
Pages (from-to)	1736-1738
Number of pages	3
Journal	Molecular Therapy
Volume	25
Issue number	8
DOIs	https://doi.org/10.1016/j.ymthe.2017.05.021
Publication status	Published or Issued - 2 Aug 2017

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics
Pharmacology
Drug Discovery

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10.1016/j.ymthe.2017.05.021

Cite this

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title = "Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9",

abstract = "CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.",

author = "Fatwa Adikusuma and Nicole Williams and Frank Grutzner and James Hughes and Paul Thomas",

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AU - Adikusuma, Fatwa

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AU - Grutzner, Frank

AU - Hughes, James

AU - Thomas, Paul

PY - 2017/8/2

Y1 - 2017/8/2

N2 - CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.

AB - CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.

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M3 - Letter

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JO - Molecular Therapy

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Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9

Abstract

ASJC Scopus subject areas

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