The activation of eukaryotic initiation factor (eIF)2B by growth factors in PC12 cells requires MEK/ERK signalling

Miranda Kleijn, Christopher G. Proud

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Epidermal and nerve growth factors (EGF and NGF) activate protein synthesis and initiation factor eIF2B in rat phaeochromocytoma (PC12) cells. The activation of protein synthesis by EGF or NGF depends upon extracellular regulated kinase kinase (MEK)/extracellular regulated kinase signalling. Here we show that PD98059, an inhibitor of MEK activation, blocks the activation of eIF2B by EGF or NGF. It is known that eIF2B activity can be inhibited by phosphorylation at Ser535 in its ε-subunit by glycogen synthase kinase (GSK)-3. We find that inactivation of GSK-3 by EGF or NGF is blocked by PD98059. However, neither EGF nor NGF caused a detectable change in phosphorylation of Ser535 of eIF2Bε. Thus, the EGF- and NGF-induced activation of eIF2B in PC12 cells involves regulatory mechanisms distinct from dephosphorylation of the GSK-3 site. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)262-265
Number of pages4
JournalFEBS Letters
Issue number3
Publication statusPublished or Issued - 7 Jul 2000
Externally publishedYes


  • Eukaryotic initiation factor (eIF)2B
  • Extracellular regulated kinase
  • Glycogen synthase kinase-3

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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