Abstract
A method for the rapid isolation and purification of proteoglycan by using solutions of LiBr for extraction and density gradient centrifugation is described. The effect of 0.5 M KOH on isolated proteoglycan has been studied by using NaB3H4 to reduce and label the chondroitin sulphate chains released. This study has established: that at least 95% of the chondroitin sulphate chains are attached to the proteoglycan by alkali labile bonds between xylose and serine; that random degradation of the chondroitin sulphate chains does not occur to any significant extent; and that the method is convenient for the determination of polysaccharide number average molecular weights.
Original language | English |
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Pages (from-to) | 457-470 |
Number of pages | 14 |
Journal | Biochemical Journal |
Volume | 133 |
Issue number | 3 |
DOIs | |
Publication status | Published or Issued - 1973 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology