The alkaline cleavage and borohydride reduction of cartilage proteoglycan

H. C. Robinson, J. J. Hopwood

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A method for the rapid isolation and purification of proteoglycan by using solutions of LiBr for extraction and density gradient centrifugation is described. The effect of 0.5 M KOH on isolated proteoglycan has been studied by using NaB3H4 to reduce and label the chondroitin sulphate chains released. This study has established: that at least 95% of the chondroitin sulphate chains are attached to the proteoglycan by alkali labile bonds between xylose and serine; that random degradation of the chondroitin sulphate chains does not occur to any significant extent; and that the method is convenient for the determination of polysaccharide number average molecular weights.

Original languageEnglish
Pages (from-to)457-470
Number of pages14
JournalBiochemical Journal
Issue number3
Publication statusPublished or Issued - 1973

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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