The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment

Jon Paul S. Powers; Morgan M. Martin; Danika L. Goosney; Robert E.W. Hancock

doi:10.1128/AAC.50.4.1522-1524.2006

The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment

Jon Paul S. Powers
, Morgan M. Martin
, Danika L. Goosney
, Robert E.W. Hancock

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

The horseshoe crab peptide polyphemusin I possesses high antimicrobial activity, but its mechanism of action is as yet not well defined. Using a biotin-labeled polyphemusin I analogue and confocal fluorescence microscopy, we showed that the peptide accumulates in the cytoplasm of wild-type Escherichia coli within 30 min after addition without causing substantial membrane damage.

Original language	English
Pages (from-to)	1522-1524
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	50
Issue number	4
DOIs	https://doi.org/10.1128/AAC.50.4.1522-1524.2006
Publication status	Published or Issued - Apr 2006
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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10.1128/AAC.50.4.1522-1524.2006

Cite this

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title = "The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment",

abstract = "The horseshoe crab peptide polyphemusin I possesses high antimicrobial activity, but its mechanism of action is as yet not well defined. Using a biotin-labeled polyphemusin I analogue and confocal fluorescence microscopy, we showed that the peptide accumulates in the cytoplasm of wild-type Escherichia coli within 30 min after addition without causing substantial membrane damage.",

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AU - Hancock, Robert E.W.

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AB - The horseshoe crab peptide polyphemusin I possesses high antimicrobial activity, but its mechanism of action is as yet not well defined. Using a biotin-labeled polyphemusin I analogue and confocal fluorescence microscopy, we showed that the peptide accumulates in the cytoplasm of wild-type Escherichia coli within 30 min after addition without causing substantial membrane damage.

UR - https://www.scopus.com/pages/publications/33645780418

U2 - 10.1128/AAC.50.4.1522-1524.2006

DO - 10.1128/AAC.50.4.1522-1524.2006

M3 - Article

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AN - SCOPUS:33645780418

SN - 0066-4804

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JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 4

ER -

The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment

Abstract

ASJC Scopus subject areas

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