The BlastGen study: a randomized controlled trial of blastocyst media supplemented with granulocyte-macrophage colony-stimulating factor

Ryan D Rose, Michael F Barry, Emma V Dunstan, Siu Man Yuen, Lyndal P Cameron, Emma J Knight, Robert J Norman, M Louise Hull

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    7 Citations (Scopus)


    RESEARCH QUESTION: Does Embryogen®/BlastGen™ culture medium improve live birth rates compared with standard culture medium for women undergoing IVF and intracytoplasmic sperm injection (ICSI) with poor prognosis.

    DESIGN: Randomized clinical trial. A total of 100 couples undergoing IVF/ICSI were randomly allocated to having their inseminated oocytes incubated in either Embryogen®/BlastGen™ sequential culture media or standard Cleavage/Blastocyst sequential culture media for 5 days ( Identifier: NCT02305420).

    RESULTS: No statistically significant difference in live birth rate was found between the control group and the Embryogen®/BlastGen™ group (17 [34%] versus 11 [22%], respectively) (OR 0.55; 95% CI 0.22 to 1.32; P = 0.18). After adjustment for maternal age, body mass index and fertilization procedure, the blastulation rate reduced (40.6 ± 26.5 versus 24.6 ± 26.7; RR 0.70, CI 0.52 to 0.95; P < 0.05), and grade of the embryo transferred (OR 0.35, CI 0.16 to 0.77; P < 0.01) when Embryogen®/BlastGen™ medium was used.

    CONCLUSION: A significant reduction in day-5 embryo outcome parameters was found using Embryogen®/BlastGen™ compared with standard medium, and insufficient evidence of a difference in pregnancy outcomes. Taking into consideration the small samples size, study limitations and strict inclusion criteria of this single-centre study, further research is needed to determine the efficacy of Embryogen®/BlastGen™ medium in couples undergoing IVF/ICSI.

    Original languageEnglish
    Pages (from-to)645-652
    Number of pages8
    JournalReproductive BioMedicine Online
    Issue number5
    Publication statusPublished or Issued - May 2020


    • Adult
    • Culture Media/chemistry
    • Embryo Culture Techniques/methods
    • Embryo Transfer/methods
    • Embryonic Development/physiology
    • Female
    • Fertilization in Vitro/methods
    • Granulocyte-Macrophage Colony-Stimulating Factor/analysis
    • Humans
    • Male
    • Pregnancy
    • Pregnancy Outcome
    • Pregnancy Rate
    • Sperm Injections, Intracytoplasmic/methods
    • Treatment Outcome

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