The effect of high-fat diet-induced obesity on the expression of nutrient chemosensors in the mouse stomach and the gastric ghrelin cell

Maria Nunez-Salces, Hui Li, Stewart Christie, Amanda J. Page

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10 Citations (Scopus)


The stomach is the primary source of the orexigenic and adiposity-promoting hormone, ghrelin. There is emerging evidence on the nutrient-mediated modulation of gastric ghrelin secretion. However, limited information is available on gastric nutrient-sensing mechanisms in high-fat diet (HFD)-induced obesity. This study investigated the impact of HFD-induced obesity on the expression of nutrient chemosensors in mouse stomach, particularly ghrelin cells. Male C57BL/6 mice were fed either a standard laboratory diet (SLD) or HFD for 12 weeks. The expression of ghrelin, enzymes involved in ghrelin production (PC1/3, GOAT) and nutrient chemosensors (CD36, FFAR2&4, GPR93, CaSR, mGluR4 and T1R3) was determined by quantitative RT-PCR in the mouse corpus and antrum. Immunohistochemistry assessed the protein expression of CaSR and ghrelin in the corpus and antrum. Antral mRNA levels of CaSR and PC1/3 were increased in HFD compared to SLD mice, while mRNA levels of all other nutrient chemosensors examined remained unchanged. CaSR immunolabelling was observed in the gastric antrum only. Nearly 80% of antral ghrelin cells expressed CaSR, with a similar cell density and co-expression in SLD and HFD mice. In conclusion, HFD-induced obesity increased CaSR mRNA expression in mouse antrum. However, the high antral co-expression of CaSR and ghrelin was unaltered in HFD compared to SLD mice.

Original languageEnglish
Article number2493
Pages (from-to)1-14
Number of pages14
Issue number9
Publication statusPublished or Issued - Sept 2020


  • CaSR
  • Ghrelin
  • High-fat diet
  • Nutrient sensing
  • Obesity
  • Stomach

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

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