The effect of the length of a malarial epitope on its antigenicity and immunogenicity in an epitope presentation system using the Pseudomonas aeruginosa outer membrane protein OprF as the carrier

Rebecca S.Y. Wong, Robert E.W. Hancock

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This study showed that the antigenicity of a malarial epitope increased with the length of the epitope when inserted at positions aa26 (amino acid position 26) and aa196, but not at aa213, of the Pseudomonas aeruginosa major outer membrane protein OprF (326 amino acids). Immunization studies showed that a 19-aa epitope was significantly more immunogenic than a 7-aa epitope when inserted at aa26 of OprF, while neither an 11- nor 19-aa epitope fused to the C-terminus of glutathione S-transferase was immunogenic.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalFEMS Microbiology Letters
Volume140
Issue number2-3
DOIs
Publication statusPublished or Issued - 1 Jul 1996
Externally publishedYes

Keywords

  • Antigen expression
  • Immune response
  • Malarial epitope
  • OprF

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this