The effects of formula feeding on physiological and immunological parameters in the gut of neonatal rats

K. L. Tooley; G. S. Howarth; R. N. Butler; K. A. Lymn; I. A. Penttila

doi:10.1007/s10620-008-0513-6

The effects of formula feeding on physiological and immunological parameters in the gut of neonatal rats

K. L. Tooley, G. S. Howarth, R. N. Butler, K. A. Lymn, I. A. Penttila

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A unique model of formula feeding in the neonatal rat was utilized to investigate the effects of an enterally delivered artificial milk formula on clinically relevant immunological and biological characteristics in the gut, compared to naturally reared pups. Hooded Wistar rat pups were randomly allocated to two treatment groups: formula-fed (FF) or naturally suckled (NS). A flexible silastic intra-gastric cannula was surgically implanted into the FF pups, through which an artificial rat milk supplement was continuously delivered from day 4 to day 10 of life. Rat pups were sacrificed at 10 days of age. Body weight, small intestinal weight, mucosal CD8⁺ cell numbers, and ileal lactase activity in FF animals were significantly decreased compared to their NS counterparts (P < 0.05). Numbers of eosinophils, mucosal mast cells, CD4⁺ T-cells, ileal villus height and gastric emptying times were significantly increased in FF pups (P < 0.05). We have developed a new rat model of artificial feeding which possesses important immunological and biological similarities to the premature human infant.

Original language	English
Pages (from-to)	1432-1439
Number of pages	8
Journal	Digestive Diseases and Sciences
Volume	54
Issue number	7
DOIs	https://doi.org/10.1007/s10620-008-0513-6
Publication status	Published or Issued - Jul 2009
Externally published	Yes

Keywords

Breast milk
Formula milk
Immuno-regulation
Intestine
Necrotizing enterocolitis
Nutrition
Prematurity

ASJC Scopus subject areas

Physiology
Gastroenterology

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10.1007/s10620-008-0513-6

Cite this

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title = "The effects of formula feeding on physiological and immunological parameters in the gut of neonatal rats",

abstract = "A unique model of formula feeding in the neonatal rat was utilized to investigate the effects of an enterally delivered artificial milk formula on clinically relevant immunological and biological characteristics in the gut, compared to naturally reared pups. Hooded Wistar rat pups were randomly allocated to two treatment groups: formula-fed (FF) or naturally suckled (NS). A flexible silastic intra-gastric cannula was surgically implanted into the FF pups, through which an artificial rat milk supplement was continuously delivered from day 4 to day 10 of life. Rat pups were sacrificed at 10 days of age. Body weight, small intestinal weight, mucosal CD8+ cell numbers, and ileal lactase activity in FF animals were significantly decreased compared to their NS counterparts (P < 0.05). Numbers of eosinophils, mucosal mast cells, CD4+ T-cells, ileal villus height and gastric emptying times were significantly increased in FF pups (P < 0.05). We have developed a new rat model of artificial feeding which possesses important immunological and biological similarities to the premature human infant.",

keywords = "Breast milk, Formula milk, Immuno-regulation, Intestine, Necrotizing enterocolitis, Nutrition, Prematurity",

author = "Tooley, {K. L.} and Howarth, {G. S.} and Butler, {R. N.} and Lymn, {K. A.} and Penttila, {I. A.}",

note = "Funding Information: Pregnant Hooded Wistar dams were acquired from the Institute of Medical and Veterinary Science (IMVS), Gilles Plains, Adelaide. Dams were housed individually with an environmental temperature of 25°C with a 12-h light:dark cycle in the Animal Care Facility of the Children, Youth, and Women{\textquoteright}s Health Service (CYWHS; Women{\textquoteright}s and Children{\textquoteright}s Hospital Campus). Approval was obtained by the Animal Ethics Committee of the CYWHS and complied with the National Health and Medical Research Council (Australia) Code of Practice for Animal Care in Research and Teaching (2004).",

year = "2009",

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doi = "10.1007/s10620-008-0513-6",

language = "English",

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AU - Butler, R. N.

AU - Lymn, K. A.

AU - Penttila, I. A.

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N2 - A unique model of formula feeding in the neonatal rat was utilized to investigate the effects of an enterally delivered artificial milk formula on clinically relevant immunological and biological characteristics in the gut, compared to naturally reared pups. Hooded Wistar rat pups were randomly allocated to two treatment groups: formula-fed (FF) or naturally suckled (NS). A flexible silastic intra-gastric cannula was surgically implanted into the FF pups, through which an artificial rat milk supplement was continuously delivered from day 4 to day 10 of life. Rat pups were sacrificed at 10 days of age. Body weight, small intestinal weight, mucosal CD8+ cell numbers, and ileal lactase activity in FF animals were significantly decreased compared to their NS counterparts (P < 0.05). Numbers of eosinophils, mucosal mast cells, CD4+ T-cells, ileal villus height and gastric emptying times were significantly increased in FF pups (P < 0.05). We have developed a new rat model of artificial feeding which possesses important immunological and biological similarities to the premature human infant.

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KW - Breast milk

KW - Formula milk

KW - Immuno-regulation

KW - Intestine

KW - Necrotizing enterocolitis

KW - Nutrition

KW - Prematurity

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ER -

The effects of formula feeding on physiological and immunological parameters in the gut of neonatal rats

Abstract

Keywords

ASJC Scopus subject areas

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