The expression of GLAST and GLT1 in a transient cerebral ischemia mongolian gerbil model

Yanling Shen, Huiling Lu, Runnan Xu, Haibo Tian, Xuewei Xia, Fiona H. Zhou, Liping Wang, Jianghui Dong, Liyuan Sun

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Purpose: Excitatory amino acid transporters (EAATs) have an indispensable function in the reuptake of extracellular glutamate. To investigate the relationship and the expression of neuronal and astrocytic markers after brain ischemia, the temporal profile of glial EAATs in both peripheral and core regions of the cortex was examined. Methods: Transient common carotid artery occlusion was used to induce unilateral transient forebrain ischemia of Mongolian gerbils, and post-ischemic brains (6 h to 2 w) were collected and prepared for immunohistochemical and Western blotting analysis of glutamine synthetase (GS), GLT-1, GLAST, S100β, and NeuN, and for Alizarin red staining of calcium deposits. Results: The expression of GLAST and GLT-1 were significantly escalated at 6 h both in the core and periphery regions, while reduced from 12 h to 2 w in the core region post-ischemia. GS-positive cellsincreasedat6hbothinthecoreandperiphery regions, while the density of Alizarin red-positive cells increased and peaked at 12 h in the ischemic cortex. The density of S100β-positive cells decreased in the ischemic core and increased in the periphery region. Immunofluorescence staining showed that S100β and TUNEL double-positive cells increased at 12 h in the core region. Conclusion: The results of GLT-1 and GLAST expression in the cortex indicate that their up-regulation was time-dependent and occurred in the acute post-ischemia period, whereas their down-regulation was region-dependent and it is involved in the pathological progress of nerve cell and glial cell death, and has a series of cascade reactions.

    Original languageEnglish
    Pages (from-to)789-800
    Number of pages12
    JournalNeuropsychiatric Disease and Treatment
    Volume16
    DOIs
    Publication statusPublished or Issued - 2020

    Keywords

    • Astrocyte
    • Forebrain ischemia
    • Glutamate transporter
    • Mongolian gerbil
    • Neuronal death

    ASJC Scopus subject areas

    • Psychiatry and Mental health
    • Biological Psychiatry

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