TY - JOUR
T1 - The Lon Protease Is Essential for Full Virulence in Pseudomonas aeruginosa
AU - Breidenstein, Elena B.M.
AU - Janot, Laure
AU - Strehmel, Janine
AU - Fernandez, Lucia
AU - Taylor, Patrick K.
AU - Kukavica-Ibrulj, Irena
AU - Gellatly, Shaan L.
AU - Levesque, Roger C.
AU - Overhage, Joerg
AU - Hancock, Robert E.W.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/11/7
Y1 - 2012/11/7
N2 - Pseudomonas aeruginosa PAO1 lon mutants are supersusceptible to ciprofloxacin, and exhibit a defect in cell division and in virulence-related properties, such as swarming, twitching and biofilm formation, despite the fact that the Lon protease is not a traditional regulator. Here we set out to investigate the influence of a lon mutation in a series of infection models. It was demonstrated that the lon mutant had a defect in cytotoxicity towards epithelial cells, was less virulent in an amoeba model as well as a mouse acute lung infection model, and impacted on in vivo survival in a rat model of chronic infection. Using qRT-PCR it was demonstrated that the lon mutation led to a down-regulation of Type III secretion genes. The Lon protease also influenced motility and biofilm formation in a mucin-rich environment. Thus alterations in several virulence-related processes in vitro in a lon mutant were reflected by defective virulence in vivo.
AB - Pseudomonas aeruginosa PAO1 lon mutants are supersusceptible to ciprofloxacin, and exhibit a defect in cell division and in virulence-related properties, such as swarming, twitching and biofilm formation, despite the fact that the Lon protease is not a traditional regulator. Here we set out to investigate the influence of a lon mutation in a series of infection models. It was demonstrated that the lon mutant had a defect in cytotoxicity towards epithelial cells, was less virulent in an amoeba model as well as a mouse acute lung infection model, and impacted on in vivo survival in a rat model of chronic infection. Using qRT-PCR it was demonstrated that the lon mutation led to a down-regulation of Type III secretion genes. The Lon protease also influenced motility and biofilm formation in a mucin-rich environment. Thus alterations in several virulence-related processes in vitro in a lon mutant were reflected by defective virulence in vivo.
UR - http://www.scopus.com/inward/record.url?scp=84868706066&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0049123
DO - 10.1371/journal.pone.0049123
M3 - Article
C2 - 23145092
AN - SCOPUS:84868706066
SN - 1932-6203
VL - 7
JO - PloS one
JF - PloS one
IS - 11
M1 - e49123
ER -