The Src/ABL kinase inhibitor dasatinib (BMS-354825) inhibits function of normal human T-lymphocytes in vitro

Stephen Blake, Timothy P. Hughes, Graham Mayrhofer, A. Bruce Lyons

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

Dasatinib (BMS-354825) is a Src/ABL tyrosine kinase inhibitor currently approved for the treatment of chronic myeloid leukemia. Dasatinib has increased potency against ABL compared to the current therapy imatinib, and is effective in many cases where disease is resistant to imatinib. Dasatinib also inhibits many Src-family tyrosine kinases. We have demonstrated in this study that dasatinib is able to block the function of normal human T-lymphocytes in vitro at clinically relevant concentrations. T-cell functions including proliferation, activation and cytokine production were all uniformly inhibited in the presence of dasatinib. We also demonstrated inhibition of TCR signalling through Src-family kinase LCK, and predicted that inhibition of LCK and other kinases involved in T-cell signalling by dasatinib is responsible for the suppression of T-cell function. These findings raise the concern about potential T-cell inhibition in patients taking dasatinib, and suggest a possible application for the treatment of T-cell mediated immune disorders.

Original languageEnglish
Pages (from-to)330-339
Number of pages10
JournalClinical Immunology
Volume127
Issue number3
DOIs
Publication statusPublished or Issued - Jun 2008

Keywords

  • Immune suppression
  • Leukemia
  • Signal transduction
  • T-cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this