Towards the commercialization of Botryococcus braunii for triterpenoid production

Khalid A. Al-Hothaly, Eric M. Adetutu, Brian H. May, Mohamed Taha, Andrew S. Ball

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Botryococcus braunii can accumulate unusually high levels of triterpenoid hydrocarbons making it a potential source of high value chemicals. However, its commercial application is hampered by its slow growth and lack of large-scale studies of triterpenoid hydrocarbon production. This study investigated hydrocarbon production in two race B of B. braunii strains, Overjuyo-3 and Kossou-4, at 25 °C in 500 L open tanks under artificial lighting in modified BG11 medium over 60 days. Maximum growth was reached by 40 days with Overjuyo-3 producing more biomass (3.05 g L−1) than Kossou-4 (2.55 g L−1). However, Kossou-4 produced more oil (0.75 g L−1) and triterpenoid hydrocarbons (C30–C34; 50 % of oil weight) compared to 0.63 g L−1 of oil in Overjuyo-3 with triterpenoid hydrocarbons making up 29 % of oil weight. This research demonstrates for the first time that large-scale production of high value triterpenoid hydrocarbon for commercial application is feasible with Kossou-4 strain.

Original languageEnglish
Pages (from-to)1415-1418
Number of pages4
JournalJournal of Industrial Microbiology and Biotechnology
Issue number10
Publication statusPublished or Issued - 26 Oct 2015
Externally publishedYes


  • Biomass
  • Botryococcus braunii
  • Large scale
  • Race B
  • Triterpenoid hydrocarbon

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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