Transcriptional variation is associated with differences in shoot sodium accumulation in distinct barley varieties

Shanika L. Amarasinghe, Nathan S. Watson-Haigh, Caitlin Byrt, Richard James, J. Qiu, Oliver Berkowitz, J. Whelan, Stuart J. Roy, Matthew Gilliham, Ute Baumann

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Soil salinity causes large productivity losses for agriculture worldwide. Barley has been identified as one of the more salt tolerant staple crops compared to wheat and rice. Identification of genes and allelic variations underlying various salt tolerance mechanisms in barley will be a practical contribution towards the development of cereal lines with greater salinity tolerance. Here, RNA from six barley varieties with varying leaf blade and sheath Na+ accumulation following salt (NaCl) treatment were sequenced. Differential gene expression analysis, variant calling and gene co-regulatory network analysis was conducted to identify potential molecular components underlying the shoot Na+ phenotypes. We identified novel alleles of HKT1;5 that could be responsible for high Na+ accumulation in blade and sheath. Furthermore, through statistical modelling of gene expression, a Na+/H+ Exchanger (NHX) gene was identified as a candidate for high sheath Na+ accumulation. Through co-expression networks, we discovered expression pattern variation for genes related to terpenoid phenylpropanoid and flavonoid metabolism amongst the six varieties. The gene candidates identified in this study provide us with targets of interest for future characterisation of molecular mechanisms that may contribute to salt stress tolerance in barley.

Original languageEnglish
Article number103812
JournalEnvironmental and Experimental Botany
Volume166
DOIs
Publication statusPublished or Issued - Oct 2019
Externally publishedYes

Keywords

  • HKT
  • Hordeum vulgare cultivars
  • NHX

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Plant Science

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