Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

Melissa J. Churchill; Paul R. Gorry; Daniel Cowley; Luxshimi Lal; Secondo Sonza; Damian F.J. Purcell; Katherine A. Thompson; Dana Gabuzda; Justin C. McArthur; Carlos A. Pardo; Steven L. Wesselingh

doi:10.1080/13550280600748946

Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

Melissa J. Churchill
, Paul R. Gorry
, Daniel Cowley
, Luxshimi Lal
, Secondo Sonza
, Damian F.J. Purcell
, Katherine A. Thompson
, Dana Gabuzda
, Justin C. McArthur
, Carlos A. Pardo
, Steven L. Wesselingh

Research output: Contribution to journal › Article › peer-review

182 Citations (Scopus)

Abstract

The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.

Original language	English
Pages (from-to)	146-152
Number of pages	7
Journal	Journal of NeuroVirology
Volume	12
Issue number	2
DOIs	https://doi.org/10.1080/13550280600748946
Publication status	Published or Issued - Apr 2006
Externally published	Yes

Keywords

Astrocyte
HIV-1
HIVD
HIVE
Macrophage
Provirus

ASJC Scopus subject areas

Neurology
Clinical Neurology
Cellular and Molecular Neuroscience
Virology

Access to Document

10.1080/13550280600748946

Cite this

Churchill, M. J., Gorry, P. R., Cowley, D., Lal, L., Sonza, S., Purcell, D. F. J., Thompson, K. A., Gabuzda, D., McArthur, J. C., Pardo, C. A., & Wesselingh, S. L. (2006). Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues. Journal of NeuroVirology, 12(2), 146-152. https://doi.org/10.1080/13550280600748946

@article{86a679b4331445258e765853ebc4009f,

title = "Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues",

abstract = "The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.",

keywords = "Astrocyte, HIV-1, HIVD, HIVE, Macrophage, Provirus",

author = "Churchill, \{Melissa J.\} and Gorry, \{Paul R.\} and Daniel Cowley and Luxshimi Lal and Secondo Sonza and Purcell, \{Damian F.J.\} and Thompson, \{Katherine A.\} and Dana Gabuzda and McArthur, \{Justin C.\} and Pardo, \{Carlos A.\} and Wesselingh, \{Steven L.\}",

year = "2006",

month = apr,

doi = "10.1080/13550280600748946",

language = "English",

volume = "12",

pages = "146--152",

journal = "Journal of NeuroVirology",

issn = "1355-0284",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "2",

}

TY - JOUR

T1 - Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

AU - Churchill, Melissa J.

AU - Gorry, Paul R.

AU - Cowley, Daniel

AU - Lal, Luxshimi

AU - Sonza, Secondo

AU - Purcell, Damian F.J.

AU - Thompson, Katherine A.

AU - Gabuzda, Dana

AU - McArthur, Justin C.

AU - Pardo, Carlos A.

AU - Wesselingh, Steven L.

PY - 2006/4

Y1 - 2006/4

N2 - The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.

AB - The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.

KW - Astrocyte

KW - HIV-1

KW - HIVD

KW - HIVE

KW - Macrophage

KW - Provirus

UR - https://www.scopus.com/pages/publications/33746901642

U2 - 10.1080/13550280600748946

DO - 10.1080/13550280600748946

M3 - Article

C2 - 16798676

AN - SCOPUS:33746901642

SN - 1355-0284

VL - 12

SP - 146

EP - 152

JO - Journal of NeuroVirology

JF - Journal of NeuroVirology

IS - 2

ER -

Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this