Yeast RNA extract suppresses human osteoclast resorption in vitro

Melissa D. Cantley, K. D. Rainsford, David R. Haynes

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: The aim of this short study was to test the combinations of RNA extracts (both the connective tissue extracts—cartilage and synovia along with yeast extract) found in natural ribonucleotide extract Osteochondrin S (OST) on human osteoclast formation and activity in vitro. Methods: In vitro human osteoclasts were treated with the RNA extracts (cartilage, synovia and yeast) at concentrations equivalent to those in OST starting from day 7 of the culture. A tartrate resistant acid phosphatase stain (TRAP) was used to indicate osteoclast formation and activity assessed by determining area of dentine resorption. Results: The combination of all components as is found in OST suppressed both osteoclast formation and activity. The yeast extract suppressed osteoclast activity at similar levels to that observed with all components combined. Conclusions: Our findings indicate that yeast RNA extracts found in OST may be the key component responsible for suppression of osteoclast activity.

Original languageEnglish
Pages (from-to)571-576
Number of pages6
JournalInflammopharmacology
Volume25
Issue number5
DOIs
Publication statusPublished or Issued - 1 Oct 2017

Keywords

  • Bone resorption
  • Osteochondrin S
  • Osteoclasts
  • RNA extract
  • Yeast extract

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Pharmacology (medical)

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